Fungal fermentation systems include yeast and filamentous fungal cells based on cell morphology and culture conditions. Yeast fermentation is the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications, including humanized antibodies. Yeast fermentation has been used in the production of chemical precursors, or even wastewater processing. In addition, yeasts' uses also extend to other products of high interest such as the generation of fuel from vegetable sources. Filamentous fungal fermentation has gained increasing attention because of the diversity of microbial products and robust production of some of the most value-added commodities.
Yeast expression systems allow efficient production of secreted and intracellular targets. In addition, yeast provides the advantage of post-translational modifications, which may be beneficial for some recombinant proteins. Various host cells and vectors are available for yeast expression. We also have some robust secretion signal sequences to ensure the highest secretion of proteins. Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. Various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi.
Codon optimization, gene synthesis, subclone gene of interest into expression vector
Transforming fungal strains
Screening and strain creation
Expression evaluation and optimization
Pilot-scale protein expression and purification
Large-scale protein expression and purification (culture volume up to 10,000L)
Process Development
With years of experience in microbial fermentation, one of our core areas of expertise is the development of large-scale processes for recombinant protein expression in microbial hosts. Now customers can flexibly choose an option that fits their requirements, from codon optimization to gene synthesis to small-scale testing and scale-up, together with protein purification and characterization.
Service Options for Fermentation Projects
Our experts have utilized microbial systems to express a wide variety of proteins.
Product Types
Other Options for Fermented End-products
Fungal Strain Options
Fermentation Plants
2-5 L fermenters, incubation chambers, master cell bank, etc.
Cutting edge technology focused on versatility for all types of development projects (up to 2,000 L fermenter).
Different fermentation chamber capacities. Auxiliary tanks and continuous sterilizers. Distillation columns.
Choose Your Configuration
Creative Biogene offers a diverse array of configurations in solid, liquid, and powder formats to deliver on your off-the-shelf and contract fermentation needs.
Packaging sizes range from small and ready-to-use to bulk super sacks. Powders are also available in water-soluble packets for fast, hassle-free dosing.
Liquids are available in quarts to tanker loads and every packaging size in between. We offer non-formulated liquids for custom formulations and fully formulated liquids and concentrates for further formulation or dilution.
Dissolution rates depend on the solid's chemistry, size, aspect ratio, and the temperature and flow of the placement environment. We provide diverse extruded solid shapes in sizes suited to institutional as well as municipal and industrial applications.
Confirm
Creative Biogene has an unparalleled track record in working with many different types of products and providing highly complex fermentation services. We have extensive experience with processes developed internally, as well as those transferred into our facilities for scale up. Our licensed facilities are prepared to meet preclinical to marketed production needs.
If you are interested in our services, please contact us for more details.
