Yeasts provide attractive expression platforms. They combine ease of genetic manipulations and the option for a simple fermentation design of a microbial organism with the capabilities of an eukaryotic organism to secrete and to modify a protein according to a general eukaryotic scheme. A range of yeast species has been developed for various applications. Commonly used strains are Pichia pastoris, Saccharomyces cerevisiae, and occasionally Hensenula polymorpha. Creative Biogene is at the forefront of protein expression with years of experiences. Equipped with a team of professional scientists, we are capable providing our clients with a variety of customized services from concept to product to meet all your needs.
Advantages
Fast Growth
Easy Genetic Manipulation
Correct Protein Processing
Post-translational Modifications
High Expression Levels
Easy Maintenance
Easy Scale-up
Inexpensive Growth Media
The general wokflow of a protein production includes gene expression, posttranslational modifications, protein secretion and downstream processing, each of steps requiring detailed design. Our yeast expression platform comprises an extensive collection of plasmids and host strains. We can provide excellent construct design strategies to obtain high-quality recombinant proteins. Various yeast expression systems and diversified flexible strategies can meet your sundry requirements for protein production.
Pichia pastoris Expression System
The methylotrophic yeast Pichia pastoris is recognized as an excellent expression system for heterologous protein production, especially when it comes to the production of complex proteins which require typical eukaryotic post-translational modifications or contain disulfide bridges.
Saccharomyces cerevisiae Expression System
Saccharomyces cerevisiae is a member of non-methylotrophic yeasts. It has the advantages of bacterial systems growing fast and can also perform post-translational modifications and secretion to drop the cost of post-fermentation in vitro purification and modification. It is an important microbial system in heterologous protein expression.
Hansenula polymorpha Expression System
The methylotrophic yeast Hansenula polymorpha, known as a non-conventional yeast, is commonly employed as an expression platform, and used for production of recombinant proteins, enzymes, vaccines, and biopharmaceuticals. As H. polymorpha is much more thermostable than P. pastoris, thus having a potential in producing proteins studied for crystallography.
Other Options for Yeast Expression System
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To better enable producing proteins with desirable properties, such as the right amount and type of glycosylation, variants of each S. cerevisiae and P. pastoris have been developed. Genetic engineering of secretion factors, such as the alpha-mating factor, a signal peptide within the N-terminus of proteins produced by P. pastoris, has helped increase the secretion of recombinant proteins. Different approaches, such as yeast strain selection, gene codon optimization, promoter strength selection, and other strategies, may help achieve functional proteins at high yields.
Scalable Expression
Using the yeast expression system for small scale-up to fermentation-scale production helps ensure you have the flexibility to produce the protein quantity you need for your application.
Production Capabilities
We possess a mature fermentation platform consisting of large fermentation tanks, bioreactors, and large-scale purification systems, which enables us to provide high-purity and kilogram level products.
Creative Biogene provides you high-quality and considerate yeast expression protein services. We specialize in yeast gene expression and purification, which can effectively save your cost and time.
If you are interested in our services, please contact us for more details.
