Thermoplasma Cultivation

Thermoplasma cells are polymorphic, varying in shape from spherical (0.3–2μm) to filamentous structures. It reproduces by budding. Its cells lack a true cell wall, but are surrounded by a single three-layer membrane, about 10-12nm thick. The genus is not motile. Colonies on solid medium containing 1.2% ionic agar, up to about 0.3 mm in diameter; these colonies are somewhat flat, coarsely granular, and dark brown. Some of them exhibit a typical "fried egg" appearance with a zona pellucida around the perimeter. Grow in liquid base containing 0.02% (NH₄)2SO₄, 0.05% MgSO₄, 0.025% CaCl₂·H₂O, 0.3% KH₂PO₄, 0.1% yeast extract and 1.0% glucose. At concentrations below 0.2%, cell harvest was proportional to the concentration of yeast extract; no growth was observed on the ether extract fraction of yeast. During the cultivation process, sterols are not required. Thermoplasma are strictly aerobic bacteria. The optimum temperature for its growth is about 59°C. Grow well between pH 0.96 and 3.0; pH optimum is about 1-2. Thermoplasma is a true saprophytic bacteria that has been isolated from a pile of burned cinders and acidic hot springs.

Thermoplasma Culture Service

Creative Biogene offers customizable Thermoplasma strain culture services. This service can help you obtain Thermoplasma cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Thermoplasma acidophilam

Figure 1. Thermoplasma acidophilum. (From microbewiki.kenyon.edu)

Thermoplasma acidophilam has the same characteristics as its genus.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

• Fermentation, centrifugation and filtration upstream process equipment;
• Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
• From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
• Recycling and Downstream Equipment;
• Waste treatment equipment.

Why Choose Us?

The culture of Thermoplasma requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.