Pullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharification and increase glucose or maltose yield. This product is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is widely used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc.