Enzyme Commission Number
EC 3.4.17.2
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
protaminase; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase
Function
A zinc enzyme formed from procarboxypeptidase B. Isolated from cattle, pig and dogfish pancreas and other sources, including skin fibroblasts and adrenal medulla. In peptidase family M14 (carboxypeptidase A family).
Applications
Research Use
Storage
Tris/HCl, 33 mmol/l; ZnCl2, 0.1 mmol/l; pH 7.5-8.5 at +25°C
Production Methods
Fermentation
Reaction
Preferential release of a C-terminal lysine or arginine amino acid
Specification
On customer requests
Description
Carboxypeptidase B, recombinant, is intended to use in highly regulated production processes at pharmaceutical companies. Carboxypeptidase B is a widely used metalloprotease, typically isolated from pancreas of different animals, that specifically releases arginine and lysine from the C-terminus of peptides and proteins. Roche has chemically synthesized a gene encoding for the amino acid sequence of the rat Carboxypeptidase B and has transformed the gene into the expression host Pichia pastoris, which expresses the recombinant Carboxypeptidase B as active protease with identical properties compared to the native Carboxypeptidase B. The product is manufactured according to DIN EN ISO 13485. No animal-derived products are used in the fermentation, purification and final formulation. The production process is validated resulting in a very high lot-to-lot consistency. Eliminate the risk of virus contamination and the risk of animal-related cross-contamination. Rely on high purity. Minimize the risk of host cell protein contamination in your final product. Increase the safety of your production processes with robust and reproducible performance and high lot-to-lot consistency.
