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Recombinant Carboxypeptidase B from Porcine

 Product Information

Cat #
MBS-1352
CAS No.
9025-24-5
Enzyme Commission Number
EC 3.4.17.2
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
protaminase; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase
Type
Function
A zinc enzyme formed from procarboxypeptidase B. Isolated from cattle, pig and dogfish pancreas and other sources, including skin fibroblasts and adrenal medulla. In peptidase family M14 (carboxypeptidase A family).
Applications
Research Use
Storage
Redissolved in 20% glycerol, 4°C, store at -20°C for long-term preservation, Avoid multiple freeze-thaw cycles.
Storage Buffer
Shelf Life
Strains
Porcine cells
Source
Porcine
Appearance
Molecular Weight
About 35kDa (SDS-PAGE detection)
Color / Form
Instruction
Enzyme Class
Hydrolases
Production Methods
Fermentation
Activity
>180U/mg
Specific Enzyme Activity
Purity
>90% (SDS-PAGE test)
Unit Definition
One unit will catalyze 1.0 μmole of Hip-L-Arg per min at pH 7.65 at 25°C.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
Preferential release of a C-terminal lysine or arginine amino acid
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
Usage And Dosage

 Description

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

For Research Use Only.
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