Prosthecochloris Cultivation

Prosthecochloris is a spherical or arc-shaped bacterium, and there is a small protrusion on the bacterium, which performs binary fission in multiple directions. When the cell division is not completely detached, the group and the branched chain are formed, and their shape depends on the direction of the division. Bacterial cells cannot move and belong to Gram-negative bacteria. Bacterial cells contain chlorophyll C and carotenoids. All pigments are located on the egg shape vesicle, which is under the cell membrane and attached to the membrane. Bubbles are not contained in the bacterial cell.

Prosthecochloris is anaerobic bacteria. When there is hydrogen sulfide, photosynthesis can be performed, which produces spherical spherical element sulfur as an intermediate oxidation product, and precipitates the outer surface of the cell. When photosynthesis, molecular oxygen is not released. The cell suspension exhibits green due to the pigmentation of the photosynthesis.

Prosthecochloris aestaarii

The cells of Prosthecochloris aestaarii were spherical to the oval, and the cell size was 1.0-1.2 microns long and 0.5-0.7 microns wide. Each cell produces about 20 protrusions, a protrusion of 0.1-0.17 microns, a length of 0.1-0.5 microns. There is a sticky wire between cells, usually buried in a viscous cap. When the cell is not completely detached, the population and branched chain can be formed. Their shape depends on the direction of the split. The vesicle containing pigment is mainly located in the protrusions. The color of a single cell is light green, the color of the cell suspension is green.

Figure1. Prosthecochloris aestaarii cells. (Ha PT, et al.; 2017)

Prosthecochloris aestaarii belongs to specialty Photoautotrophic bacteria, and is strictly anaerobic, and VB12 is required, and the most suitable growth pH range: 6.7-7.0.  2-5% NaC1 is required in the growth medium. It can withstand NaCl from 1 to 8%. Ultimate growth temperature: 25 ° -30 ° C. Photosynthetic electron donors: sulfide, sulfur. When the sulfide and CO are present, the acetate can be photointed.

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The culture of Prosthecochloris requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.