Product Overview
The PKA kinase activity kit is a colorimetric activity-based assay kit that can be used to detect PKA activity in cell, tissue and buffer samples. The kit contains all the reagents for the PKA assay and is capable of reading endpoints or kinetic assays in a convenient 96-well plate-based assay.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Store all components at 4°C, except active kinase at -80°C.
Instruction
RUO - Research Use Only
Shipping
Shipped on Dry Ice
Recommendation
Colorimetric detection,Activity assay
Species Reactivity
Species independent
Contents
Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications
Description
PKA is an important class of kinases known as Arg-directed kinases or AGC family kinases, including cAMP-dependent protein kinases (PKA or cAPK), cGMP-dependent protein kinases (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position 3 relative to the phosphorylated serine or threonine2-4. The second messenger cyclic AMP (cAMP) activates PKA in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation2.PKA is implicated in many cellular processes, including regulation of other protein kinases, regulation of intracellular calcium concentration, and regulation of transcription. The transcriptional response to increased cAMP is achieved through activation of cAMP response element binding protein (CREB), cAMP response element regulator (CREM) and activating transcription factor 1 (ATF1). Each of these transcription factors contains a kinase-inducible structural domain containing a conserved PKA phosphorylation site.
