This kit is designed for the specific and exclusive in vitro quantification of both Norovirus genotypes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the Norovirus genome. Two sets of primers and probe have been designed directed against each genotype which form distinct genetic linkages. The primers and probe sequences in this kit have 100% homology with a broad range of Norovirus sequences based on a comprehensive bioinformatics analysis.