Product Overview
Biochemical assays for microbial pathogen detection work on the basis of polymerase chain reaction (PCR) or reverse-transcriptase PCR.
Features
Robust Lyophilized qPCR master mix;
Positive copy number quantification;
Highly specific detection profile;
High priming efficiency;
Accurate controls.
Technology
A real-time polymerase chain reaction (real-time PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.
Type
Molecular detection for animal pathogen, RUO.
Applications
Agricultural Microbiology
Storage
• Store at -20°C
• Repeated thawing and freezing (>3x) should be avoided
• Cool all reagents during the working steps
Strains
Naegleria fowleri
Instruction
Molecular detection for human pathogen, RUO.
Shipping
Shipping at room temperature
Specification
Standard and Custom Specifications
