Enzyme Commission Number
EC 3.2.1.17
Product Overview
Lysozyme has a broad antibacterial spectrum, is effective against both Gram-positive bacteria and Gram-negative bacteria, and is also effective against some viruses, and is safe and non-toxic to the human body without side effects. Therefore, it is currently widely used in food, feed and medicine and other fields.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
muramidase; globulin G; mucopeptide glucohydrolase; globulin G1; N,O-diacetylmuramidase; lysozyme g; L-7001; 1,4-N-acetylmuramidase; mucopeptide N-acetylmuramoylhydrolase; PR1-lysozyme
Function
cf. also EC 3.2.1.14 chitinase.
Applications
Research Use
Production Methods
Fermentation
Unit Definition
Lysozyme can hydrolyze the cell wall of bacteria, causing the dissolution of Micrococcus luteus and reducing the absorbance value of the solution. Under the conditions of pH 6.2 and temperature 25°C, the amount of lysozyme required to cause an absorbance change of 0.001 per minute at 450 nm using the suspension of Micrococcus luteus is one enzyme activity unit (U).
WARNINGS
Avoid multiple freeze-thaw cycles.
Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.
Shipping
Timely shipping by optional means
Reaction
Hydrolysis of (1→4)-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Specification
On customer requests
Description
Lysozymes, also known as muramidase or N-acetylmuramide glycanhydrolase, are glycoside hydrolases. These are enzymes (EC 3.2.1.17) that damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the macrophages and the polymorphonuclear neutrophils (PMNs). Large amounts of lysozyme can be found in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure, making them part of the same family. In humans, the lysozyme enzyme is encoded by the LYZ gene.
