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Human Rotavirus A Nucleic Acid Detection Kit

 Product Information

Cat #
CBMN-DG019
CAS No.
Enzyme Commission Number
Product Overview
Biochemical assays for microbial pathogen detection work on the basis of polymerase chain reaction (PCR) or reverse-transcriptase PCR.
Features
Robust Lyophilized RT-qPCR master mix;
Highly specific detection profile;
High priming efficiency;
Accurate controls.
Method
Technology
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo biopsies.
Synonyms
Type
Molecular detection for human pathogen, RUO.
Function
Applications
Clinical Microbiology
Storage
• Store at -20°C
• Repeated thawing and freezing (>3x) should be avoided
• Cool all reagents during the working steps
Storage Buffer
Shelf Life
Strains
Rotavirus
Source
Appearance
Molecular Weight
Color / Form
Instruction
Molecular detection for human pathogen, RUO.
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipping at room temperature
Formula
Reaction
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard and Custom Specifications
Substrates
Concentration
Usage And Dosage

 Description

This kit is designed for the in vitro quantification of Human Rotavirus A genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design. The dynamics of genetic variation means that new sequence information may become available after the initial design. Due to very high sequence homology between the viruses, the primers and probe in this kit will also detect rotavirus A isolates from a range of non-human hosts.

For Research Use Only.
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