This kit is designed for the in vitro quantification of HRV16 genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the HRV16 genome. The kit has been designed for the specific and exclusive in vitro quantification of this subtype and does not detect other human rhinovirus strains. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.