This kit is designed for the in vitro quantification of H1 genomes. The primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design. The dynamics of genetic variation means that new sequence information may become available after the initial design. The primers have very high homology with all reference sequences submitted since 2000 and therefore have a very broadest detection profile. These primers will also detect most historical sequences for this subtype. Due to the inherent instability of RNA viral genomes, it is not possible guarantee the quantification of all clinical isolates.