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HAT Activity Assay Kit

 Product Information

Cat #
MBNK-20
CAS No.
Enzyme Commission Number
Product Overview
The HAT activity assay kit provides a rapid assay for HATase activity in a variety of samples using acetyl CoA and H4 histone peptides as substrates. In this assay, the HAT enzyme catalyzes the transfer of acetyl groups from acetyl CoA to histone peptides, resulting in two products - acetylated peptide and CoA-SH - which then serve as essential coenzymes for the production of NADH,which can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Type
Function
Applications
Research Use
Storage
Long term storage at -80°C.
Storage Buffer
Shelf Life
Strains
Source
Appearance
Molecular Weight
Color / Form
Instruction
RUO - Research Use Only
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipped on Dry Ice
Formula
Reaction
Recommendation
Colorimetric detection,Activity assay
Species Reactivity
Contents
2x HAT Assay Buffer,HAT Substrate I,HAT Substrate II,NADH Generating Enzyme,NE (Nuclear Extract 4 mg/ml),HAT Reconstitution Buffer
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard Specifications
Substrates
Concentration
Usage And Dosage

 Description

Histone acetyltransferases (HATs) are epigenetic enzymes that install acetyl groups onto lysine residues of cellular proteins such as histones, transcription factors, nuclear receptors, and enzymes. HATs have been shown to play a role in diseases ranging from cancer and inflammatory diseases to neurological disorders, both through acetylations of histone proteins and non-histone proteins.HATs mediate many different biological processes including cell-cycle progression, dosage compensation, repair of DNA damage, and hormone signaling. Aberrant HAT function is correlated with several human diseases including solid tumors, leukemias, inflammatory lung disease, viral infection, diabetes, fungal infection, and drug addiction.

For Research Use Only.
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