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Goat Meat Speciation Nucleic Acid Detection Kit

 Product Information

Cat #
CBMN-DG211
Product Overview
Biochemical assays for microbial pathogen detection work on the basis of polymerase chain reaction (PCR) or reverse-transcriptase PCR.
Features
Robust Lyophilized qPCR master mix;
Highly specific detection profile;
High priming efficiency;
Accurate controls.
Technology
A real-time polymerase chain reaction (real-time PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA and (6) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.
Type
Molecular detection for Meat/Fish Speciation
Applications
Food Microbiology
Storage
• Store at -20°C
• Repeated thawing and freezing (>3x) should be avoided
• Cool all reagents during the working steps
Instruction
Molecular detection for Meat/Fish Speciation
Shipping
Shipping at room temperature
Specification
Standard and Custom Specifications

 Description

This kit provides a method for detecting Capra hircus mitochondrial DNA in food samples. The kit is supplied in a multiplex format enabling both species-specific and ‘universal animal’ detection in a single well. The relative signals produced by each of these targets will allow the user to calculate speciation percentage with minimal sample usage.

For Research Use Only.
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