Functional Microbes

Sulfur bacteria

Microbes exist in all kinds of environments in nature. Some microbes with special functions have received widespread attention due to the importance or particularity of their functions. These microbes with special functions are called functional microbes, such as ammonia-oxidizing bacteria, sulfur bacteria, and nitrifying bacteria, etc.

Microbes play an important role in the biogeochemical cycle, directly affecting the health of animals and plants and the development of agriculture. In the past few decades, scientists have been trying to clarify issues related to microbial diversity and ecosystem functions. At present, a variety of techniques such as physiology, biochemistry, molecular and microscopy can be used to identify and study various functions of microbial communities.

Technical Process

  • The process of functional microbe high-throughput sequencing analysis.

The process of functional microbe high-throughput sequencing analysis - Creative Biogene

Sample Type and Detection Type

  • The requirements of DNA sample: total DNA ≥ 500ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The main detection types are bacteria, fungi, and protozoa, etc.

What Can We Offer?

According to customer needs, we can design PCR universal primers for functional genes or housekeeping genes of microbes, and carry out personalized library construction and analysis. Such methods can be used for detailed classification and analysis of various species, even subspecies, and combined with environmental data, which can be used for classification and evolution studies of certain types of microbes.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
PCoA
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
PCA
RDA/CCA
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages 

  • Our solutions cover almost all aspects of microbiology, allowing you to enjoy a one-stop experience.
  • Simplified workflows with comprehensive bioinformatics analysis and high quality publishable data delivery.
  • We can customize the service and combine it with a quick turnaround at an affordable cost.

Creative Biogene has many years of experience in the field of functional microbes. Our team is composed of professional scientists, researchers and technicians. We can not only provide customers with standardized analysis content that has undergone rigorous testing and evaluation, but also can provide a full range of personalized customized solutions according to the project requirements. You can choose us with confidence, we anticipate your presence.

If you are interested in our services, please contact us for more details.

Reference

  1. Zhang Z, et al. (2018). "Stability of aerobic granular sludge under condition of low influent C/N ratio: Correlation of sludge property and functional microorganism." Bioresource technology. 270: 391-399.
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