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Caspase-9 Colorimetric Assay Kit

 Product Information

Cat #
MBNK-14
CAS No.
Enzyme Commission Number
Product Overview
The Caspase-9 colorimetric assay kit is used for the rapid detection of Caspase-9 activity in various samples. The assay principle is based on the spectrophotometric detection of the chromophore p-nitroaniline (p-NA) after cleavage from the labeled substrate LEHD-p-NA.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Type
Function
Applications
Research Use
Storage
Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.Long term storage at -20°C.
Storage Buffer
Shelf Life
Strains
Source
Appearance
Molecular Weight
Color / Form
Instruction
RUO - Research Use Only
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipped on Blue Ice
Formula
Reaction
Recommendation
Colorimetric detection,Activity assay
Species Reactivity
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl LEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard Specifications
Substrates
Concentration
Usage And Dosage

 Description

Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling.Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form.Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change.Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.

For Research Use Only.
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