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Caspase-3 Colorimetric Assay Kit

 Product Information

Cat #
MBNK-11
CAS No.
Enzyme Commission Number
Product Overview
The Caspase-3 colorimetric assay kit is used for the rapid detection of Caspase 3 in various samples. The assay principle is based on the fact that Caspase 3 hydrolyzes acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA), releasing the p-nitroanilide (pNA) fraction. The concentration of pNA released from the substrate can be calculated by measuring the absorbance value at 405 nm.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Type
Function
Applications
Research Use
Storage
Store Lysis Buffer, Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.Long term storage at -20°C.
Storage Buffer
Shelf Life
Strains
Source
Appearance
Molecular Weight
Color / Form
Instruction
RUO - Research Use Only
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipped on Blue Ice
Formula
Reaction
Recommendation
Colorimetric detection,Activity assay
Species Reactivity
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-pNA (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard Specifications
Substrates
Concentration
Usage And Dosage

 Description

Caspase-3 is a cysteine-aspartate protease that plays a key role in apoptosis and is widely used as a biomarker of apoptosis. caspase-3 is sheared into two fragments with molecular weights of 17 kDa (p17) and 12 kDa (p12) during apoptosis and translocated to the nucleus. caspase-3 is activated by shearing Caspase-3 is involved in the degradation of Huntington's protein. caspase-3 is involved in the cleavage of amyloid precursor protein, which is associated with neuronal death in Alzheimer's disease.

For Research Use Only.
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