Product Overview
The Calcineurin cellular activity assay kit is a colorimetric assay based kit that contains all the reagents needed to measure cytosolic calcium phosphatase (PP2B) phosphatase activity, plus human recombinant calcium phosphatase included as a positive control.The RII phosphopeptide substrate, supplied with this kit is the most efficient and selective peptide known for calcineurin. The detection of free-phosphate released is based on the classic malachite green assay.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Store all components except the microtiter plate at 80°C for the highest stability. The calcineurin enzyme component SE163-9090 must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be quickly refrozen by placing at -80°C. To minimize the number of freeze/thaw cycles, aliquot the calcineurin into separate tubes and store at -80°C. Long term storage at -80°C.
Instruction
RUO - Research Use Only
Shipping
Shipped on Dry Ice
Recommendation
Colorimetric detection
Contents
Calcineurin enzyme, Calmodulin (human, recombinant), Substrate (RII phosphopeptide), 2x assay buffer, EGTA Buffer, Lysis Buffer, Protease inhibitor cocktail, Biomol Green™ Reagent, Phosphate standard, Okadaic acid, Desalting column and resin, ½-Volume microplate
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications
