Bile Acid Detection

Bile acid (BA) is the main organic component of bile and is a collective term for several steroid acids with similar structures. The main configuration of bile acid has two sides, hydrophilic and hydrophobic, which make the molecules have the characteristics of interfacial active molecules and can reduce the surface tension between the oil and water phases. According to the source, bile acids can be divided into primary bile and secondary bile acids. Primary bile acids are directly synthesized by hepatocytes, and secondary bile acids are the products of intestinal bacterial powder and enterohepatic circulation.

Application of Bile Acid Detection

Bile acids mainly exist in the enterohepatic circulatory system. They play an important role in promoting lipid emulsification and enhancing pancreatic lipolysis，directly participate in lipid and glucose metabolism. Bile acids are beneficial to food digestion and nutrient absorption through recycling, and they are closely related to obesity and immune response. In clinical and disease diagnosis, bile acids are significant in the study of hepatobiliary and gastrointestinal diseases. Bile acid detection can assist you in the above researches.

Our Service Process

Service Offering

Creative Biogene’s bile acid detection service provides results including,

• Raw data
• Experimental steps
• Related mass spectrometry parameters
• Quantitative or qualitative information of the identified substance
• Mass spectrum picture
• Bioinformatics analysis report

Our bioinformatics analysis covers a wide range including transition pair selection, standard curve drawing, data quality control, quantitative results of target small molecules, comparative group analysis and phenotypic analysis, etc.

Sample Type

Feces, blood, urine, bile, yeast and other microbes, etc.

Detection platform

Instrument platform: LC-MS-MS and MRM.

Our LC-MS-MS technology achieves the quantitative and qualitative analysis of more than 90 bile acids and related substance standards, combined with bile acid isotope standards. Among them, 95% of the substances involved are quantitatively and clearly analyzed using isotope standards, which significantly improve the accuracy of the results.

Turnaround Time

In general, our turnaround time is 3-5 weeks depending on the size of your project.

Advantages of Our Services 

• With high throughput, it can obtain quantitative information on a variety of small molecules at the same time.
• The accuracy of our results is high because of the advanced detection platform.
• Our exclusive kit is specifically designed for bile acids and can achieve precise quantification.
• We can combine the demands of customers, and negotiate to determine the content of services.

Creative Biogene has excellent microbiology experts, well-established metabolome detection platforms, and professional bioinformatics analysis to provide you with a one-stop service in bile acid detection. Our services include sample collection, metabolite extraction, pre-processing, detection and analysis.

If you are interested in our services, please contact us for more details.