Archangeium Cultivation

Archangeium vegetative cells are elongated, pointed, flexible rods. Archangeium has no sporangia, and its fruiting bodies are irregular in shape, swollen or brain-like, and are composed of intestinal-like coiled or intertwined clumps. The bacteria do not have a clear mucus wall. Microsporangia of Archangium are short rods, oval or spherical, refractive or optically dense. Vegetative colonies do not etch or corrode agar media. Colonies can adsorb Congo red.

Figure 1. Archangeium sp. fruiting bodies. (Kathrin I. Mohr. 2018)

Archangeium Culture Service

Archangeium gephyra

The vegetative cells of archangium gephyra have a characteristic shape. The cells are slightly pointed, and the ends are slightly swollen circles, with a size of 0.4-0.7×6-15 microns. Fruiting bodies vary widely in size and shape, and are usually composed of an irregular brain-like mass, or an elongated mass with a bulging surface. Its texture is firm, and color is variable. In reflected light, the fruiting bodies are pale rose or reddish flesh-colored to orange; bluish-purple when viewed on a dark background. When viewed in transmitted light, the fruiting bodies are yellow to light red. Fruit bodies are orange or red when dried. The interior of the fruiting body is formed by the accumulation of tubular objects, which are periodically blocked by transverse walls, but the transverse walls do not completely cut the spore mass from one side to the other. When the tube was pressurized between the slide and the coverslip, it broke apart into many irregular, round clumps 15-30 microns in diameter. In the clumps, microsporangia are arranged in bundles.

The microsporangia of archangeium gephyra are spherical, oval or short bean-shaped and rod-shaped. Optically dense and refractive, the size is 1.0-2.0 × 1.5-2.8 microns.

On agar media, vegetative colonies are thin and have many radial ridges or concentric folds. The margins are thin and have tongue-like projections. Where cells accumulate, colonies are brown. The production of mucus is moderate and easy to cut. Growth was weak in Casitone-Mg²⁺ liquid culture; in shaking culture, the growths formed some clinging orange globules. Adding 0.1-0.2% of agar in the medium, its growth condition has been greatly improved.

This bacterium grows easily on a complex medium which commonly used for culturing non-fibrinolytic myxobacteria. The study found that archangeium gephyra cannot reduce nitrate, does not produce contact enzymes and oxidase, and can hydrolyze esculin, starch, cheese, gelatin, Tween 80, saccharol acetate, RNA and DNA.

Archangeium gephyra is aerobic bacteria. The optimum growth pH is 7.5. The growth temperature range is 18°-40°C, and the optimum temperature is 18°-32°C. The bacteria can be resistant to 10 units of penicillin, but are sensitive to 10 micrograms of neomycin, 5 micrograms of streptomycin, 10 micrograms of tetracycline, 10 micrograms of ceramycin, 10 micrograms of kanamycin or 5 micrograms of erythromycin.

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The culture of Archangeium requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.