Airborne Microecology

Airborne microbes.

Airborne microecology is a microbial ecosystem that is closely related to other environmental biospheres, and directly affects the human respiratory system and skin. The status of microbes in the air and human health are closely related. With the extensive application of DNA sequencing and metagenomics in the study of microbial diversity, the community structure of airborne microbes has been gradually recognized. The types and quantities of microbes in the air vary with environmental conditions such as region, altitude, season, climate, etc.

The air has a strong transmission capacity. Once pathogens enter the human body, it may turn the air into a transmission medium and cause the epidemic of infectious diseases. The Noval Coronavirus is mainly spread by airborne droplets at close range. Therefore, conducting airborne microbial testing to determine the nature and degree of air pollution caused by bacteria or viruses is of great significance for the prevention and control of infectious diseases as well as environmental hygienic supervision.

Technical Process

  • The process of airborne microbe high-throughput sequencing analysis.

The process of airborne microbe high-throughput sequencing analysis - Creative Biogene

  • The process of airborne microbe PCR-DEEG analysis.

The process of airborne microbe PCR-DEEG analysis - Creative Biogene

Sample Type and Detection Type

  • The airborne microbial samples are collected using biofilm.
  • The requirements of DNA sample: total DNA ≥ 500ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The detection types are bacteria, fungi, viruses, etc.

What Can We Offer?

  • Isolate, cultivate and identify airborne microbes and explore their sources.
  • Study the abundance and community structure of airborne microbes in different environments.
  • Study the relationship between airborne microbes and the environment.
  • Explore the function of airborne microbes and their relationship to human diseases

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics, microbial genome editing and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
PCoA
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
PCA
RDA/CCA
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages

  • We have a mature bioinformatics analysis platform and professional scientists.
  • We can customize the solution and combine it with a quick turnaround at an affordable cost.
  • We will feedback progress to customers in time to ensure that your requirements are met.

The study of airborne microecology is very important for the prevention of infectious diseases and the monitoring of the atmospheric environment. Creative Biogene is committed to providing the best service to accelerate the realization of customers' research goals. Our airborne microecology solution is excellent in speed, accuracy and sensitivity, the result we offer is with good reproducibility.

If you are interested in our services, please contact us for more details.

Reference

  1. Prussin A, et al. (2015). "Sources of airborne microorganisms in the built environment." Microbiome. 3.1: 78.
For Research Use Only.
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